Fascination About attune flow cytometer
Fascination About attune flow cytometer
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Facts-enriched characterization of mobile populations. Morphological facts from pictures can increase to the richness of flow cytometry details. For instance, the figure displays an if not conventional apoptosis assay employing Annexin V and PI, introducing cell imaging to characterize cells in Every population to expose morphologically unique functions. These insights could not are already acquired from multiplex staining on your own.
As samples are acquired around the Attune CytPix Flow Cytometer, the superior-pace brightfield digicam captures and retailers illustrations or photos of detected situations at a fee around six,000 photographs for every 2nd, based on the flow charge and graphic dimension. For better overall flexibility, Attune Cytometric Software allows you to adjust the picture seize frequency as required and select a certain gate for graphic capture, if required.
Mix acoustic focusing technological know-how with large-parameter Investigation for adaptable spectral unmixing and conventional workflows.
Find out new insights into your experimental info by visualizing populations making use of mixed fluorescence and morphology facts. Our program interface wizards allow downsampling, concatenation (merged information) and dimensionality instruments which are developed into your application for an intuitive, streamlined details Assessment expertise.
"An image isn't really pretty worth one thousand dots, but it surely really really helps to see photos. It is great to discover visuals that correlate with staining. One example is, AnnexinV-good cells which are morphologically distinctive than healthful cells, or CD14+ monocytes that are larger and much more textured than CD3+/CD4+/CD14- lymphocytes.
Right here, a highly trained consumer has gated singlets confidently. Immediately after analyzing the guide singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate has more than four% aggregates.
Pair your Attune flow cytometer by having an autosampler for enhanced workflow performance, and increase robotic integration for maximum throughput.
Our researchers will structure panels for you as often times you require—totally free, no product or service buy essential.
Early indications of this contamination had in the beginning been dismissed as particles. Backgating to find out new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also enables you to use https://chancekxkvg.wikipublicist.com/5132573/flow_cytometer_a_revolutionary_tool_for_cell_analysis morphological attributes to discover fascinating subpopulations that will not be apparent from flow cytometry facts by yourself.
Maybe most significantly, these occasions comprise cells of clearly various phenotypes which may lead to incorrect conclusions relating to double constructive situations (especially in unusual populations).
Audio waves help exact cell alignment, resulting in Outstanding info quality and ~10X more quickly sample flow fees.
Application Observe: Automatic graphic analysis lessens person-to-consumer variability in flow cytometry gating strategies
Developed-in dimensionality reduction applications greater resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to confirm predicted inhabitants resolution in lysed complete human blood. Person can see situations from Every cluster for visual confirmation of mobile phenotypes.
No clean/no lyse leukocyte Examination with imaging. Cells ended up obtained from 24-hour-outdated blood by dilution in one mM EDTA ( Skilled UX/UI allows customers to prepare AI designs for expanded purposes. (A) The “Educate Design” attribute provides annotation instruments and true-time model education accuracy.
Gating on these extended parameters allows you to speedily and accurately establish populations of desire and make sure gating strategy with little or no guide overview. The Attune software presents use of state-of-the-artwork equipment Studying Investigation resources inside of an expertly created user interface, which makes it extremely convenient to use.
The Attune Xenith cytometer is usually a significant-parameter spectral analyzer, offering equally spectral unmixing and traditional flow capabilities for thorough and adaptable cell Investigation.
The Attune CytPix Flow Cytometer makes it possible for consumers to visualize samples all through acquisition, validate the precision of gating procedures, and assistance final results with trustworthy documentation for publication functions.
What seemed to be a homogeneous inhabitants is actually more diverse—an insight that should be deemed when interpreting outcomes.